Low-Cost Methods for Molecular Characterization of Mutant Plants

Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols

by Bradley J. Till, Joanna Jankowicz-Cieslak, Owen A. Huynh, Mayada M. Beshir, Robert G. Laport, Bernhard J. Hofinger

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Book Description

This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.

This open book is licensed under a Creative Commons License (CC BY-NC). You can download Low-Cost Methods for Molecular Characterization of Mutant Plants ebook for free in PDF format (1.0 MB).

Book Details

Publisher
Springer
Published
2015
Pages
42
Edition
1
Language
English
ISBN13
9783319162584
ISBN10
3319162586
ISBN13 Digital
9783319162591
ISBN10 Digital
3319162594
PDF Size
1.0 MB
License
CC BY-NC

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