This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
This open book is licensed under a Creative Commons License (CC BY-NC). You can download Low-Cost Methods for Molecular Characterization of Mutant Plants ebook for free in PDF format (1.0 MB).
This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular charac...
In this offers a methodological response to recent efforts by scholars in rhetoric and composition/writing studies to account for patterns indicative of the discipline's maturation. Influenced by work on distant reading and thin description, this monograph attends to forms of knowledge newly available via computationally mined, aggregated data from...
This book offers effective, low-cost and user-friendly protocols for the pre-field selection of salt-tolerant mutants in cereal crops. It presents simple methods for measuring soil salinity, including soil sampling and the analysis of water-soluble salts, and describes a detailed, but simple, screening test for salt tolerance in rice, wheat and bar...
In the light of better and more detailed administrative databases, this book provides statistical tools for evaluating the effects of public policies advocated by governments and public institutions. Experts from academia, national statistics offices and various research centers present modern econometric methods for an efficient data-driven policy...
This book summarizes the results of 3 years of agricultural and forestry reconstructive efforts and applied research conducted directly in the affected areas of Fukushima following the Great East Japan Earthquake. It describes fast and effective revival methods and technologies from tsunami and radiation damages, demonstrated through the collaborat...
This book introduces new methods for measuring and analyzing residential segregation. It begins by placing all popular segregation indices in the "difference of group means" framework wherein index scores can be obtained as simple differences of group means on individual-level residential attainments scored from area racial composition. ...